Manual flowpath b1 or b2 buffervalveb pump a pump b common inlet a2 a1 common inlet b2 b1. The aim of this tutorial is to demonstrate the use of the ubiquitous akta systems and prepacked hitrap chromatography media for rapid, mediumscale protein purification. It will help if they get w10 with some good dev support when it comes out. Systems provide more control than manual purification because of the ability to automatically control the flow rate and monitor the progress of the purification as well as to make controlled gradients and automatically collect fractions.
The ge akta explorer chromatography systems, formally from amersham pharmacia biotech offer fast and reliable solutions for developing and optimizing any biomolecular purification scheme. Repair and pm services for akta explorers, fplc, purifier, prime plus, pilot, xflow, avant, and pure refurbished and new parts are available at reasonable prices. The amersham pharmacia akta purifier is a highly modular fast performance liquid chromatography fplc system. Achieve desired purity with ease in routine purificationsmake the most of valuable benchcold room space. Get familiarized with its use and have its instruction manual handy. Easytouse system that allows you to transition from manual to automated protein purification. Information about these options can be found in optional configurations user manual which describes the extended functions of ae. Bufferprep kit eliminates the timeconsuming manual. Once the lines have been washed with water, you will need to start a manual run before attaching your column to the system. Instrument ships complete and ready to use, and ready for optional professional installation. Repair services range between onsite services and phone support. We had the same problem on and off in he past but we could solve it just running a manual run for a few second.
Aktapurifier user guide ge healthcare life sciences manualzz. Aktaprime plus user manual 11002644 edition aa 9 1introduction this section is an introduction to aktaprime plus, the documentation included and the content in this manual. If a pump that has been washed with 20% ethanol is transferred directly to a saltcontaining buffer, the salt will precipitate. Introduction 1 aktafplc system manual 18114045 edition ae 7 1 introduction 1. Flowpath buffervalvea a1 or a2 flowpath buffervalveb b1 or b2 for each line you will use draw air from all 4 ports pump a pump b common inlet a2 a1. Gloves and protective eyewear should be worn when handling samples and reagents buffers, however it is preferable that the user remove gloves prior to entering commands via the computer keyboard or mouse. Akta laboratoryscale chromatography systems handbook. Fraction collector frac950 user manual 181956 edition ae 7 1 introduction 1. This manual is designed for operators, maintenance personnel and service engineers working with the alfa laval s 300 separator. This manual describes the operation of the aktafplc system.
Akta avant laboratory equipment pdf manual download. Call us today to learn more about the ge amersham pharmacia akta pilot, our service product offerings on the entire akta product line, a personalized pricing proposal, or how to purchase a brand new production biochromatography fplc system. Akta pure 25 new owners intro the exercise below will give a quick demonstration of how easy and intuitive the akta pure 25 will be for you in demonstrating downstream processing to your students. This set up creates a lower flow rate but at a higher pressure required for.
Ge atka purifier 10 fplc system still in its originally factory seal and available at the lab world group. The complete documentation is also available on the user documentation cd. Free download ebooks press the directional key up or down to highlight a note. Akta avant user manual protein production and purification. No uninet connection between controller and the system. Akta fplc is compatible with all solvents commonly used in protein purification. This is a modular fast performance liquid chromatography system used primarily for fast separations and purifications of proteins, peptides and nucleic acids. Back pressure increase may indicate treatment is overdue. This fplc has a capacity of up to 100 ml per minute. Bioscisolutions repairs your akta, biacore, and agilent. There should be a clean titanium filter 18112094 available in the akta supplies drawer that can be swapped out for the one being cleaned. Fraction collector frac950 boyce thompson institute. Medical equipment ge optima xr220amx operators manual. Aktapurifiertm core systems fig 1 are versatile, modular liquid chromatography.
Xray system 93 pages medical equipment ge logiq 7 basic user manual. Please change the country on your profile in order to switch to another country store. Ge healthcare akta fplc frac950 fraction collector frac950 10593 duration. Ensure that the hg lamp position and the filter is selected according to the wave. Easily share your publications and get them in front of issuus. Box monitor conductivity ph uv detector pumps injector buffers pump a. If the back pressure of a gel filtration column is high but the system is reasonable, then ask clara to show you how to. The system simplifies the transition from laboratory to full scale production. It also contains an overview of the safety instructions. Direct access to manual instructions via run data or system flow path. The akta purifier 100 upc is a more economical choice than the akta purifier 100 ideally suited for applications that require 10 mil of flow rate per minute fast and easy start to purification work preprogramme. Purifier uppurifier down mode should be operated high mode only works when unit is in. Ge akta explorer amersham pharmacia certified fplc system. Ge healthcare akta purifier 10 fplc system youtube.
Ge akta purifier 10 fplc system the lab world group. Evaluating the uv response calculate the uv response ratios in the. Fplc standard operating procedure akta purifier system page page 2 of 9 check there is sufficient volumes of buffers, mq and etoh 20% for the coming runs. Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. View and download ge akta avant user manual online.
Aktapurifier box monitor conductivity ph uv detector pumps. The ge akta purifier 10 is a flexible modular fast protein liquid chromatography system. Always keep the he inlet above the solution or close the valve. Fplc standard operating procedure akta purifier system page page 1 of 9 fplc standard operating procedure akta system ge note. Aktapurifiertm core systems fig 1 are versatile, modular. Protein purification laboratory of molecular biology. Such systems are easily modified to best suit the current and future needs of research and production laboratories. The akta purifier 100 comes preprogrammed with application protocols and method templates, making it a fast and easy start to. Monitor upc900 user manual 18112555 edition ag vii. Ge healthcare akta purifier 10 fplc system available from the lab world group. Opening the unicorn software windows for manual control and programmable features purgingpriming pumps and tubing. Ge akta purifier user manual ge akta purifier user manual. The manual injection valve is a 6port rotary valve that is manually operated to transfer the preloaded sample onto the column. Using the akta prime plus october 22, 2012 3 j e n n i f e r a r r u d a attaching the column and washing the ethanol from the column.
Pdf, ebook, free books to download, user guard, ebook, books, manual, ge, akta, purifier, user, manual. Open draw air out of buffer line go to system control window manual choose. System description, system maintenance and troubleshooting are also. Flexible and intuitive purification system to meet.
Aktafplc is a fully automated liquid chromatography system designed for research scale purification of proteins. Akta purifier 10 up to 10 mlmin flow rate at pressures of 0 to 25 mpa. Akta chromatography systems have been staple in protein purification for the past two decades and used throughout the globe. User documentation on the cd the user documentation listed in the table below is available in printed or pdf format. It also features a tunable detector, allowing for selection of a wide spectral range. July 4, different optional configurations might be akta purifier 10 manual required, this is a modular fast performance liquid chromatography system used primarily for. Aktaexplorer am ers ha m bio scie nce am er sham bio ci n a m e r s h a m b io s c ie n c a m e r s h a m b io s c ie box n c e monitor. Ge akta pilot liquid chromatography system gmi cerfied. Akta start can be combined with the frac30 fraction collector. If the separator has been delivered and installed by alfa laval as a part of a processing system, this manual should be viewed as part of the system documentation. The ge akta purifier 100 is a fast protein liquid chromatograph or fplc, which is capable of handling a wide range of wavelengths. Chromatographic techniques at flow rates and pressures compatible with modern separation media are used to produce highcaliber solutions. The proven design has been verified during development and can be userconfigured to meet specific process demands. The sample loop for the injection of small sample volumes 25.
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